Long Range Dpp Gradient Formation

From Computational Biology Group

(Difference between revisions)
Jump to: navigation, search
Line 2: Line 2:
 
<newstitle> Understanding Dpp gradient formation mechanism </newstitle>
 
<newstitle> Understanding Dpp gradient formation mechanism </newstitle>
 
     <teaser>
 
     <teaser>
In collaboration with the Basler group (University of Zurich), we developed a theoretical model allowing to understand which is the leading mechanism involved in the Dpp long range gradient formation.
+
In collaboration with the Basler group (University of Zurich), we developed a theoretical model allowing to understand which is the leading mechanism involved in the Dpp long range gradient formation. The article appeared online in [http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001111 PLoS Biology] on 26 Juli 2011.
 
+
The article appeared online in [http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001111 PLoS Computational Biology] on 26 Juli 2011.
+
 
     <date>18 October 2011 — 11:13</date>
 
     <date>18 October 2011 — 11:13</date>
 
     </teaser>
 
     </teaser>

Revision as of 15:30, 1 November 2011



Decapentaplegic (Dpp) is a key morphogen which is expressed in a stripe of cells along the anteriorposterior (A-P) boundary of the Drosophila wing imaginal discs and diffuses along the A-P axis forming, at steady-state, a "quasi exponential" profile. The mechanisms by which this profile is formed has however long been controversal and two distinct mechanisms involving Dpp receptors have been proposed: Receptor-Mediated Transcytosis (RMT) and Restricted Extracellular Diffusion (RED).

In a recent paper (for more details click here), we developed a rigorous theoretical model which involves three Dpp components: extracellular Dpp, receptor-bound Dpp and internalized Dpp. Providing a different parameter choice, this model allows to describe both the RMT and RED mechanism. Comparing our analytical model to wild-type and receptor mutant clone experimental data, we conclude that (1) the RMT mechanism is not consistent with our experimental data (2) a RED mechanism where most of the Dpp is unbound to the receptor leads to the expected Dpp profiles.


Gerald Schwank, Sascha Dalessi, Schu-Fee Yang, Ryohei Yagi, Aitana Morton de Lachapelle, Markus Affolter, Sven Bergmann, Konrad Basler
Formation of the long range Dpp morphogen gradient.
PLoS Biol.: 2011, 9(7);e1001111
[PubMed:21814489] [WorldCat.org] [DOI]