Difference between revisions of "Long Range Dpp Gradient Formation"
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<newstitle> Understanding Dpp gradient formation mechanism </newstitle> | <newstitle> Understanding Dpp gradient formation mechanism </newstitle> | ||
<teaser> | <teaser> | ||
| − | In collaboration with the Basler group (University of Zurich), we developed a theoretical model allowing to understand which is the leading mechanism involved in the Dpp long range gradient formation. | + | In collaboration with the Basler group (University of Zurich), we developed a theoretical model allowing to understand which is the leading mechanism involved in the Dpp long range gradient formation. The article appeared online in <a href="http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001111">PLoS Biology</a> on 26 July 2011. |
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| − | The article appeared online in | ||
<date>18 October 2011 — 11:13</date> | <date>18 October 2011 — 11:13</date> | ||
</teaser> | </teaser> | ||
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In a recent paper (for more details [http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001111 click here]), we developed a rigorous theoretical model which involves three Dpp components: extracellular Dpp, receptor-bound Dpp and internalized Dpp. Providing a different parameter choice, this model allows to describe both the RMT and RED mechanism. | In a recent paper (for more details [http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001111 click here]), we developed a rigorous theoretical model which involves three Dpp components: extracellular Dpp, receptor-bound Dpp and internalized Dpp. Providing a different parameter choice, this model allows to describe both the RMT and RED mechanism. | ||
| − | Comparing our analytical model to wild-type and receptor mutant clone experimental data, we conclude that | + | Comparing our analytical model to wild-type and receptor mutant clone experimental data, we conclude that |
| − | (1) the RMT mechanism is not consistent with our experimental data | + | (1) the RMT mechanism is not consistent with our experimental data |
(2) a RED mechanism where most of the Dpp is unbound to the receptor leads to the expected Dpp profiles. | (2) a RED mechanism where most of the Dpp is unbound to the receptor leads to the expected Dpp profiles. | ||
Latest revision as of 17:06, 20 August 2012
Decapentaplegic (Dpp) is a key morphogen which is expressed in a stripe of cells along the anteriorposterior (A-P) boundary of the Drosophila wing imaginal discs and diffuses along the A-P axis forming, at steady-state, a "quasi exponential" profile.
The mechanisms by which this profile is formed has however long been controversal and two distinct mechanisms involving Dpp receptors have been proposed: Receptor-Mediated Transcytosis (RMT) and Restricted Extracellular Diffusion (RED).
In a recent paper (for more details click here), we developed a rigorous theoretical model which involves three Dpp components: extracellular Dpp, receptor-bound Dpp and internalized Dpp. Providing a different parameter choice, this model allows to describe both the RMT and RED mechanism. Comparing our analytical model to wild-type and receptor mutant clone experimental data, we conclude that (1) the RMT mechanism is not consistent with our experimental data (2) a RED mechanism where most of the Dpp is unbound to the receptor leads to the expected Dpp profiles.
Gerald Schwank, Sascha Dalessi, Schu-Fee Yang, Ryohei Yagi, Aitana Morton de Lachapelle, Markus Affolter, Sven Bergmann, Konrad Basler
Formation of the long range Dpp morphogen gradient.
PLoS Biol.: 2011, 9(7);e1001111
[PubMed:21814489]
[WorldCat.org]
[DOI]