Fluorescence Activated Cell Sorting (FACS) Service
FFluorescence Activated Cell Sorting (FACS) extends gated analysis to isolate pure populations of for instance viable cells with more homogeneous characteristics than could be obtained by any other means.
If you can get the cells that interest you into a gate in your multi-parameter measurement space, you can get them into a test tube, or into the wells of a multi-well plate.
A flow cytometer is equipped for sorting by the addition of a mechanism for diverting cells from the sample stream and of electronics and/or computer hardware and software that can determine, within a few microseconds after a cell passes by the cytometer's sensors, whether the values of one or more measurement parameters fall within a range or ranges preset by the operator, and generate a signal that activates the sorting mechanism.
The selected cells can then be subjected to further biochemical analysis, observed in short- or long-term culture, or reintroduced into another biological system. The Flow Cytometry Facility of LICR@UNIL offers a cell sorting service, available to all UNIL internal and external research groups.
The current cell sorter, a FACSAria I offers capability of differentiating between 10 fluorescent parameters in addition to forward and side scatter and is located at the Epalinges site of the FCF. In order to make a reservation for cell sorting or to get more information about the Service please contact Danny Labes.
Useful information can also be found on the FAQ, Download and Instrument sections of this website!