Difference between revisions of "Long Range Dpp Gradient Formation"

 
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<newstitle> Understanding Dpp gradient formation mechanism </newstitle>
 
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In collaboration with the Basler group (University of Zurich), we developed a theoretical model allowing to understand which is the leading mechanism involved in the Dpp long range gradient formation.
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The article appeared online in [http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001111 PLoS Computational Biology] on 26 Juli 2011.
 
The article appeared online in [http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001111 PLoS Computational Biology] on 26 Juli 2011.
 
     <date>18 October 2011 — 11:13</date>
 
     <date>18 October 2011 — 11:13</date>
 
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Decapentaplegic (Dpp) is a key morphogen which is expressed in a stripe of cells along the anteriorposterior (A-P) boundary of the Drosophila wing imaginal discs and diffuses along the A-P axis forming, at steady-state, a "quasi exponential" profile.
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The mechanisms by which this profile is formed has however long been controversal and two distinct mechanisms involving Dpp receptors have been proposed: Receptor-Mediated Transcytosis (RMT) and Restricted Extracellular Diffusion (RED).
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In a recent work [ref], we developed a rigorous theoretical model which involves three Dpp components: extracellular Dpp, receptor-bound Dpp and internalized Dpp. Providing a different parameter choice, this model allows to describe both the RMT and RED mechanism.
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Comparing our analytical model to wild-type and receptor mutant clone experimental data, we conclude that
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(1) the RMT mechanism is not consistent with our experimental data
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(2) a RED mechanism where most of the Dpp is unbound to the receptor leads to the expected Dpp profiles.
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For more details click [http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001111 here] .
 
For more details click [http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001111 here] .
  

Revision as of 15:35, 18 October 2011



Decapentaplegic (Dpp) is a key morphogen which is expressed in a stripe of cells along the anteriorposterior (A-P) boundary of the Drosophila wing imaginal discs and diffuses along the A-P axis forming, at steady-state, a "quasi exponential" profile. The mechanisms by which this profile is formed has however long been controversal and two distinct mechanisms involving Dpp receptors have been proposed: Receptor-Mediated Transcytosis (RMT) and Restricted Extracellular Diffusion (RED).

In a recent work [ref], we developed a rigorous theoretical model which involves three Dpp components: extracellular Dpp, receptor-bound Dpp and internalized Dpp. Providing a different parameter choice, this model allows to describe both the RMT and RED mechanism. Comparing our analytical model to wild-type and receptor mutant clone experimental data, we conclude that (1) the RMT mechanism is not consistent with our experimental data (2) a RED mechanism where most of the Dpp is unbound to the receptor leads to the expected Dpp profiles.

For more details click here .

Schwank G, Dalessi S, Yang SF, Yagi R, de Lachapelle AM, Affolter M, Bergmann S, Basler K
Formation of the long range Dpp morphogen gradient.
PLoS Biol: 2011 Jul, 9(7);e1001111
[PubMed:21814489] [WorldCat.org: ISSN ESSN ] [DOI] ( o)