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Module #561, TG: 3, TC: 1.6, 63 probes, 63 Entrez genes, 13 conditions
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The image plot shows the color-coded level of gene expression, for the
genes and conditions in a given transcription module. The genes are on
the horizontal, the conditions on the vertical axis.
The genes are ordered according to their ISA gene scores, similarly
the conditions are ordered according to their condition scores. The
score of a gene means the «degree of inclusion» in
the module: a high score gene is essential in the module.
Condition scores can also be negative, that means that the genes of
the module are all down-regulated in the condition. Here the absolute
value of the score gives the «degree of inclusion».
The plots above and beside the expression matrix show the gene scores
and condition scores, respectively.
Note that the plot is interactive, you can see the name of the gene
and condition under the mouse cursor.
The expression matrix was normalized to have mean zero and standard
deviation one for every gene separately across all conditions
(i.e. not just for the conditions in the module).
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Under-expression is coded with green,
over-expression with red color.
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The GO tree — Biological processes
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This is one of three sections showing Gene Ontology enrichment of the
current module: in this case for biological processes.
The graph shows the hierarchy of the GO categories, their enrichment
for the current module is color coded, and the blue number beside the
category is the minus log ten p-value of the enrichment. (Calculated
using the standard hypergeometric test.) The color of the arrows code
«is a» (cyan) and «part of» relationships.
The tree was built the following way. First all GO terms with more
significant enrichment p-value than 0.05 were collected. Then all
paths from these terms to the root node of the GO tree were included
too. If a GO term is included more than once in the tree, then the
green numbers show 1) the id of the node, this makes it easier to find
other appereances of the term, and 2) the number of appearences.
Note that the same GO category might show up on the graph many
times. This is because the GO was «straightened» for this
graph, i.e. if there are more paths from a GO term to the root node of
the tree, all of them are included. The green numbers
Move the mouse cursor over the terms to get their definition. Clicking
on them takes you to the corresponding Gene Ontology web page.
If you cannot see a graph here at all, that means that there were no
significantly enriched GO categories, at the 0.05 level.
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The GO tree — Cellular Components
HELP
This is one of three sections showing Gene Ontology enrichment of the
current module: in this case for cellular components.
The graph shows the hierarchy of the GO categories, their enrichment
for the current module is color coded, and the blue number beside the
category is the minus log ten p-value of the enrichment. (Calculated
using the standard hypergeometric test.) The color of the arrows code
«is a» (cyan) and «part of» relationships.
The tree was built the following way. First all GO terms with more
significant enrichment p-value than 0.05 were collected. Then all
paths from these terms to the root node of the GO tree were included
too. If a GO term is included more than once in the tree, then the
green numbers show 1) the id of the node, this makes it easier to find
other appereances of the term, and 2) the number of appearences.
Note that the same GO category might show up on the graph many
times. This is because the GO was «straightened» for this
graph, i.e. if there are more paths from a GO term to the root node of
the tree, all of them are included. The green numbers
Move the mouse cursor over the terms to get their definition. Clicking
on them takes you to the corresponding Gene Ontology web page.
If you cannot see a graph here at all, that means that there were no
significantly enriched GO categories, at the 0.05 level.
— Click on the Help button again to close this help window.
extracellular region
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
cellular_component
The part of a cell or its extracellular environment in which a gene product is located. A gene product may be located in one or more parts of a cell and its location may be as specific as a particular macromolecular complex, that is, a stable, persistent association of macromolecules that function together.
extracellular region part
Any constituent part of the extracellular region, the space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers constituent parts of the host cell environment outside an intracellular parasite.
all
This term is the most general term possible
extracellular region part
Any constituent part of the extracellular region, the space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers constituent parts of the host cell environment outside an intracellular parasite.
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The GO tree — Molecular Function
HELP
This is one of three sections showing Gene Ontology enrichment of the
current module: in this case for molecular function.
The graph shows the hierarchy of the GO categories, their enrichment
for the current module is color coded, and the blue number beside the
category is the minus log ten p-value of the enrichment. (Calculated
using the standard hypergeometric test.) The color of the arrows code
«is a» (cyan) and «part of» relationships.
The tree was built the following way. First all GO terms with more
significant enrichment p-value than 0.05 were collected. Then all
paths from these terms to the root node of the GO tree were included
too. If a GO term is included more than once in the tree, then the
green numbers show 1) the id of the node, this makes it easier to find
other appereances of the term, and 2) the number of appearences.
Note that the same GO category might show up on the graph many
times. This is because the GO was «straightened» for this
graph, i.e. if there are more paths from a GO term to the root node of
the tree, all of them are included. The green numbers
Move the mouse cursor over the terms to get their definition. Clicking
on them takes you to the corresponding Gene Ontology web page.
If you cannot see a graph here at all, that means that there were no
significantly enriched GO categories, at the 0.05 level.
— Click on the Help button again to close this help window.
protein binding
Interacting selectively with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
molecular_function
Elemental activities, such as catalysis or binding, describing the actions of a gene product at the molecular level. A given gene product may exhibit one or more molecular functions.
MAP-kinase scaffold activity
Functions as a physical support for the assembly of a multiprotein mitogen-activated protein kinase (MAPK) complex. MAPK scaffold proteins have binding sites for MAPK pathway kinases as well as for upstream signaling proteins.
structural molecule activity
The action of a molecule that contributes to the structural integrity of a complex or assembly within or outside a cell.
binding
The selective, often stoichiometric, interaction of a molecule with one or more specific sites on another molecule.
receptor signaling complex scaffold activity
Functions to provide a physical support for the assembly of a multiprotein receptor signaling complex.
protein complex scaffold
Functions to provide a physical support for the assembly of a multiprotein complex.
all
This term is the most general term possible
protein complex scaffold
Functions to provide a physical support for the assembly of a multiprotein complex.
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GO BP test for over-representation
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List of all enriched GO categories (biological processes), at the 0.05
p-value level.
The columns:
- ExpCount is the expected count of genes in the
module annotated with the given GO term, just by chance.
- Count
is the number of genes in the module annotated with the given GO
term.
- Size is the total number of genes (in our universe)
annotated with the GO term.
Clicking on Count shows the genes that drive the
enrichment. You can also click on the individual numbers in
the Count column, to show the driving genes for that individual
GO category.
Clicking on the GO identifiers takes you to the Gene Ontology web
pages.
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| Id | Pvalue | ExpCount | Count | Size | Term |
| GO:0006955 | 1.127e-02 | 1.981 | 9
AZGP1, CYBB, DMBT1, EDA, ITGAL, LILRA5, LILRB3, ST6GAL1, TBX21 | 295 | immune response |
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GO CC test for over-representation
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List of all enriched GO categories (cellular components), at the 0.05
p-value level.
The columns:
- ExpCount is the expected count of genes in the
module annotated with the given GO term, just by chance.
- Count
is the number of genes in the module annotated with the given GO
term.
- Size is the total number of genes (in our universe)
annotated with the GO term.
Clicking on Count shows the genes that drive the
enrichment. You can also click on the individual numbers in
the Count column, to show the driving genes for that individual
GO category.
Clicking on the GO identifiers takes you to the Gene Ontology web
pages.
— Click on the Help button again to close this help window.
| Id | Pvalue | ExpCount | Count | Size | Term |
| GO:0005576 | 2.670e-06 | 4.591 | 19
ADAMTS7, AZGP1, CFHR2, COL11A2, DMBT1, EDA, F11, FGL1, IGHG1, LILRA5, LTBP4, MFNG, MSTP9, MUC5AC, NOTCH4, PGC, RLN1, ST6GAL1, VGF | 700 | extracellular region |
| GO:0044421 | 5.656e-03 | 2.538 | 10
ADAMTS7, COL11A2, F11, FGL1, LTBP4, MFNG, MUC5AC, NOTCH4, PGC, VGF | 387 | extracellular region part |
| GO:0030173 | 1.562e-02 | 0.164 | 3
MFNG, ST6GAL1, ST8SIA3 | 25 | integral to Golgi membrane |
| GO:0031228 | 2.089e-02 | 0.1837 | 3
MFNG, ST6GAL1, ST8SIA3 | 28 | intrinsic to Golgi membrane |
| GO:0009925 | 4.899e-02 | 0.07215 | 2
MYO1A, NOTCH4 | 11 | basal plasma membrane |
| GO:0045178 | 4.899e-02 | 0.07215 | 2
MYO1A, NOTCH4 | 11 | basal part of cell |
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GO MF test for over-representation
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List of all enriched GO categories (molecular function), at the 0.05
p-value level.
The columns:
- ExpCount is the expected count of genes in the
module annotated with the given GO term, just by chance.
- Count
is the number of genes in the module annotated with the given GO
term.
- Size is the total number of genes (in our universe)
annotated with the GO term.
Clicking on Count shows the genes that drive the
enrichment. You can also click on the individual numbers in
the Count column, to show the driving genes for that individual
GO category.
Clicking on the GO identifiers takes you to the Gene Ontology web
pages.
— Click on the Help button again to close this help window.
| Id | Pvalue | ExpCount | Count | Size | Term |
| GO:0005078 | 6.126e-03 | 0.01293 | 2
MAPK8IP1, MAPK8IP2 | 2 | MAP-kinase scaffold activity |
| GO:0004872 | 1.293e-02 | 3.447 | 12
ADORA1, ADRA1D, DMBT1, ITGAL, LILRA5, LILRB3, LRRN2, LTBP4, NOTCH4, OR1E2, OR7E24, PRSS7 | 533 | receptor activity |
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KEGG Pathway test for over-representation
HELP
List of all enriched KEGG pathways, at the 0.05
p-value level.
The columns:
- ExpCount is the expected count of genes in the
module annotated with the given KEGG pathway, just by chance.
- Count
is the number of genes in the module annotated with the given KEGG
pathway.
- Size is the total number of genes (in our universe)
annotated with the KEGG pathway.
Clicking on Count shows the genes that drive the
enrichment. You can also click on the individual numbers in
the Count column, to show the driving genes for that individual
KEGG pathway.
Clicking on the KEGG identifiers takes you to the KEGG web site.
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miRNA test for over-representation
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List of all enriched miRNA families, at the 0.05
p-value level.
The columns:
- ExpCount is the expected count of genes in the
module regulated by the given miRNA family, just by chance.
- Count
is the number of genes in the module regulated by the given miRNA
family.
- Size is the total number of genes (in our universe)
regulated with the given miRNA family.
Clicking on Count shows the genes that drive the
enrichment. You can also click on the individual numbers in
the Count column, to show the driving genes for that individual
miRNA family.
The miRNA regulation data was taken from the TargetScan database.
(Only the conserved sites were used for the current analysis.)
Clicking on the miRNA names takes you to the TargetScan web site.
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Chromosome test for over-representation
HELP
List of all enriched Chromosomes, at the 0.05
p-value level.
The columns:
- ExpCount is the expected number of genes in the
module on the given chromosome, just by chance.
- Count
is the number of genes in the module on the given chromosome.
- Size is the total number of genes (in our universe)
on the given chromosome.
Clicking on Count shows the genes that drive the
enrichment. You can also click on the individual numbers in
the Count column, to show the driving genes for that individual
chromosome.
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HELP
A list of all genes in the current module, in alphabetical order. The
size of the text corresponds to the gene scores.
Note that some gene symbols may show up more than once, if many
probes match the same Entrez gene.
Genes with no Entrez mapping are given separately, with their
Affymetrics probe ID.
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Entrez genes
ADAMTS7ADAM metallopeptidase with thrombospondin type 1 motif, 7 (220705_s_at), score: -0.87
ADORA1adenosine A1 receptor (216220_s_at), score: -0.87
ADRA1Dadrenergic, alpha-1D-, receptor (210961_s_at), score: -0.81
AZGP1alpha-2-glycoprotein 1, zinc-binding (217014_s_at), score: -0.76
BTNL3butyrophilin-like 3 (217207_s_at), score: -0.77
C7orf28Achromosome 7 open reading frame 28A (201974_s_at), score: -0.77
CCDC9coiled-coil domain containing 9 (206257_at), score: -0.84
CFHR2complement factor H-related 2 (206910_x_at), score: -0.72
COL11A2collagen, type XI, alpha 2 (216993_s_at), score: -0.87
CYBBcytochrome b-245, beta polypeptide (203923_s_at), score: -0.85
CYP3A4cytochrome P450, family 3, subfamily A, polypeptide 4 (205998_x_at), score: -0.85
DMBT1deleted in malignant brain tumors 1 (208250_s_at), score: -0.78
EDAectodysplasin A (211130_x_at), score: -0.98
ETNK2ethanolamine kinase 2 (219268_at), score: -0.77
F11coagulation factor XI (206610_s_at), score: -0.79
FBRSfibrosin (218255_s_at), score: -0.81
FGL1fibrinogen-like 1 (205305_at), score: -0.8
FKBP10FK506 binding protein 10, 65 kDa (219249_s_at), score: -0.96
FOLH1folate hydrolase (prostate-specific membrane antigen) 1 (217487_x_at), score: -0.71
GBA3glucosidase, beta, acid 3 (cytosolic) (219954_s_at), score: -0.73
HAB1B1 for mucin (215778_x_at), score: -0.92
HAND1heart and neural crest derivatives expressed 1 (220138_at), score: -0.69
HAO2hydroxyacid oxidase 2 (long chain) (220801_s_at), score: -0.79
IGHG1immunoglobulin heavy constant gamma 1 (G1m marker) (217039_x_at), score: -0.73
INE1inactivation escape 1 (non-protein coding) (207252_at), score: -0.77
ITGALintegrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) (213475_s_at), score: -0.78
KRT8P12keratin 8 pseudogene 12 (222060_at), score: -0.73
LILRA5leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5 (215838_at), score: -0.71
LILRB3leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 (211133_x_at), score: -0.74
LOC149478hypothetical protein LOC149478 (215462_at), score: -0.77
LOC80054hypothetical LOC80054 (220465_at), score: -0.89
LRRN2leucine rich repeat neuronal 2 (216164_at), score: -0.79
LTBP4latent transforming growth factor beta binding protein 4 (210628_x_at), score: -0.76
MAPK8IP1mitogen-activated protein kinase 8 interacting protein 1 (213013_at), score: -0.78
MAPK8IP2mitogen-activated protein kinase 8 interacting protein 2 (205050_s_at), score: -0.8
MFNGMFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (204153_s_at), score: -0.73
MOBPmyelin-associated oligodendrocyte basic protein (210193_at), score: -0.9
MSTP9macrophage stimulating, pseudogene 9 (213382_at), score: -0.7
MUC5ACmucin 5AC, oligomeric mucus/gel-forming (217182_at), score: -0.73
MYL4myosin, light chain 4, alkali; atrial, embryonic (210088_x_at), score: -0.85
MYO1Amyosin IA (211916_s_at), score: -0.83
NCRNA00092non-protein coding RNA 92 (215861_at), score: -0.78
NOTCH4Notch homolog 4 (Drosophila) (205247_at), score: -0.75
OR1E2olfactory receptor, family 1, subfamily E, member 2 (208587_s_at), score: -0.74
OR7E24olfactory receptor, family 7, subfamily E, member 24 (215463_at), score: -0.78
PGCprogastricsin (pepsinogen C) (205261_at), score: -0.77
PGK2phosphoglycerate kinase 2 (217009_at), score: -0.72
PIPOXpipecolic acid oxidase (221605_s_at), score: -0.86
PMS2L2postmeiotic segregation increased 2-like 2 pseudogene (215410_at), score: -0.69
PRSS7protease, serine, 7 (enterokinase) (217269_s_at), score: -0.95
PYHIN1pyrin and HIN domain family, member 1 (216748_at), score: -0.94
RLN1relaxin 1 (211753_s_at), score: -0.73
RPGRIP1retinitis pigmentosa GTPase regulator interacting protein 1 (206608_s_at), score: -0.75
S100A14S100 calcium binding protein A14 (218677_at), score: -0.78
SEPT5septin 5 (209767_s_at), score: -0.74
SLC26A10solute carrier family 26, member 10 (214951_at), score: -0.82
ST6GAL1ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (201998_at), score: -0.81
ST8SIA3ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3 (208064_s_at), score: -0.87
TBX21T-box 21 (220684_at), score: -0.75
TMSB4Ythymosin beta 4, Y-linked (206769_at), score: -1
TNK1tyrosine kinase, non-receptor, 1 (217149_x_at), score: -0.75
TULP2tubby like protein 2 (206733_at), score: -0.74
VGFVGF nerve growth factor inducible (205586_x_at), score: -0.91
Non-Entrez genes
Unknown, score:
HELP
Conditions in the module, given in the same order as on the expression
plot above. Red color means over-expression, green under-expression in
the given condition.
The barplot below shows the condition (sample) scores. A separate bar
is shown for each sample, its height is the corresponding score of the
sample in the module. The red and green numbers on the bars are the
sample scores expressed in percents, i.e. 100% is 1.0.
The red and green lines show the module thresholds, samples above
the red line and below the green line are included in the module.
The different experiments that were part of the study, are separated
by dashed vertical lines.
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| Id | sample | Experiment | ExpName | Array | Syndrome | Cell.line |
| ctrl a 08-03.CEL | 1 | 1 | DS-CC | hgu133a | none | DS-CC 1 |
| E-TABM-263-raw-cel-1515485931.cel | 15 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
| t21a 08-03.CEL | 4 | 1 | DS-CC | hgu133a | Down | DS-CC 4 |
| E-TABM-263-raw-cel-1515485671.cel | 2 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
| t21d 08-03.CEL | 7 | 1 | DS-CC | hgu133a | Down | DS-CC 7 |
| E-TABM-263-raw-cel-1515486071.cel | 22 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
| E-TABM-263-raw-cel-1515485871.cel | 12 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
| E-TABM-263-raw-cel-1515485831.cel | 10 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
| E-TABM-263-raw-cel-1515486131.cel | 25 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
| E-TABM-263-raw-cel-1515485711.cel | 4 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
| E-TABM-263-raw-cel-1515485951.cel | 16 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
| E-TABM-263-raw-cel-1515486111.cel | 24 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
| E-TABM-263-raw-cel-1515485651.cel | 1 | 6 | Cycle | hgu133a2 | none | Cycle 1 |
© 2008-2010 Computational Biology Group, Department of Medical Genetics,
University of Lausanne, Switzerland
