Top-bottom differences in retinal vascular properties

Differences in tortuosity between top and bottom retinal vasculature

Jonathan NICOLET-DIT_FÉLIX, Bertille BOURG, Louis HEAU

Supervisor: Sacha BORS

To find out if there was a difference in tortuosity between top and bottom segments, we first put together a method to separate the blood vessels, then we computed their tortuosity and determined there was a very small positive asymmetry.

Presentation of the project: why is it important/relevant?

Retina fundus images are a type of picture taken by a special camera through the iris. They allow us to visualise the vasculature of the retina. This is a very convenient non-invasive way to obtain an insight of the body's vascular features, and the ultimate goal is to be able to use these pictures as a proxy for different diseases linked to vasculature such as ocular diseases (diabetic retinopathy, glaucoma) but also more general cardiovascular diseases like stroke, coronary heart disease or hypertension.

But to make the link between diseases and the retinal vasculature, we need to extract features from the fundus images such as (the number of bifurcation, the length, the evolution of the diameter...). One of the features that has recently drawn attention is tortuosity: how sinuous a blood vessel segment is compared to the direct length from its two extremities. Tortuosity is an important feature to study because it has potential links with cardiovascular diseases.

What is unknown is if there is a difference between the tortuosity in the top of the retina vs the bottom for arteries or veins.

Basic concepts

Indeed, when we look at a fundus image what is striking is that there are two large vascular networks above and below a line between the fovea and the optical disc. Arteries and veins are two stacked layers with different biological functions, so we compare them separately in the analysis. But we have these two sets. are they different ? especially for tortuosity ? Should we compute them separately in the analysis or can we use a "global tortuosity ? "

The dataset we will be using for the analysis is the UK biobank dataset, which contains for one patient their genome sequence, and a huge lot of various traits, including the fundus images from their retinas. with this dataset we have a great number of images. These images can be automatically analysed with a special python package based on machine learning program that's being developed called vascX (LWNET), that can extract features from the retina fundus. The extracted features are stored in a retina object, containing many things such as a representation of the retina as a graph with edges and nodes, and the position of important features such as the optical disc (root of all the vasculature supplying the retinal cells ) and the fovea (focal point of an image on the retina, composed of the majority of the cones)