Difference between revisions of "Cellophane"
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In collaboration with the group of Sophie Martin from DMF (UNIL), we developed Cellophane, an ImageJ plugin that semi-automatically quantifies fluorescent protein concentration profiles along the cell cortex. This plugin enabled the quantification of hundreds of profiles of two key regulators of the fission yeast cell cycle, Pom2 and Cdr2. The data analysis, along with other experimental evidence, showed that two important functions of Pom1, deciding when and where to divide, require distinct levels of Pom1. Lower Pom1 level are sufficient for division positioning, but higher levels are required to delay mitotic entry until the proper size is reached. The paper has been published in <a href="http://www.landesbioscience.com/journals/cc/article/27411/"> Cell Cycle </a>. | In collaboration with the group of Sophie Martin from DMF (UNIL), we developed Cellophane, an ImageJ plugin that semi-automatically quantifies fluorescent protein concentration profiles along the cell cortex. This plugin enabled the quantification of hundreds of profiles of two key regulators of the fission yeast cell cycle, Pom2 and Cdr2. The data analysis, along with other experimental evidence, showed that two important functions of Pom1, deciding when and where to divide, require distinct levels of Pom1. Lower Pom1 level are sufficient for division positioning, but higher levels are required to delay mitotic entry until the proper size is reached. The paper has been published in <a href="http://www.landesbioscience.com/journals/cc/article/27411/"> Cell Cycle </a>. | ||
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Revision as of 14:53, 9 December 2013
Introduction
Cellophane is an ImageJ plugin for the semi-automated quantification of a protein profile along the cell membrane. It allows for the quantification along two channels. The plugin also has a manual mode which has a lower throughput but allows for higher precision.
Download
The plugin is available under a GPL license and the code is available here. This zip files contains two .java files containing the semi-automated and manual versions of Cellophane, as well as the necessary java library file.
You can also download the short manual. If you have additonal questions, you can contact Micha
Credits
This plugin was developed by Micha Hersch in collaboration with the lab of Sophie Martin at the University of Lausanne, in particular with Olivier Hachet. Sascha Dalessi helped testing the software. It uses the imagescience library written by Erik Meijering.
